Xov Xwm

Mimicking physiological tej yam kev mob pab cov kws tshawb fawb nrhiav tau hlau binders

Cov kws tshawb fawb tau tsim ib txoj hauv kev los txheeb xyuas cov molecules me me uas khi hlau ions. Hlau ions yog qhov tseem ceeb hauv biology. Tab sis kev txheeb xyuas cov molecules - thiab tshwj xeeb tshaj yog cov molecules me - cov hlau ions cuam tshuam nrog tuaj yeem nyuaj.

Txhawm rau cais cov metabolites rau kev txheeb xyuas, cov txheej txheem metabolomics cov txheej txheem siv cov kuab tshuaj organic thiab pHs qis, uas tuaj yeem ua rau cov hlau tsis sib xws. Pieter C. Dorrestein ntawm University of California San Diego thiab cov neeg ua hauj lwm xav ua kom cov complexes ua ke rau kev soj ntsuam los ntawm kev ua raws li cov xwm txheej hauv zej zog. Tab sis yog tias lawv siv cov kev mob physiological thaum lub sij hawm sib cais ntawm cov molecules, lawv yuav tsum tau rov kho qhov kev sib cais ntawm txhua tus mob uas lawv xav sim.

Hloov chaw, cov kws tshawb fawb tau tsim ob-theem mus kom ze uas qhia txog lub cev nqaij daim tawv ntawm kev sib cais ntawm cov pa chromatographic thiab kev tshuaj ntsuam xyuas huab hwm coj (Nat. Chem. 2021, DOI: 10.1038/s41557-021-00803-1). Ua ntej, lawv tau cais cov tshuaj lom neeg siv cov pa ua haujlwm siab ua kua chromatography. Tom qab ntawd lawv tau kho cov pH ntawm cov dej ntws tawm ntawm kab chromatographic kom ua raws li cov xwm txheej ntawm lub cev, ntxiv cov hlau ions, thiab tshuaj xyuas qhov sib xyaw nrog huab hwm coj spectrometry. Lawv khiav qhov kev tshuaj ntsuam ob zaug kom tau txais huab hwm coj ntawm cov molecules me me nrog thiab tsis muaj hlau. Txhawm rau txheeb xyuas qhov twg cov molecules khi hlau, lawv tau siv txoj hauv kev suav uas siv cov duab siab tshaj plaws los txiav txim siab kev sib txuas ntawm cov spectra ntawm kev khi thiab tsis sib xws.

Ib txoj hauv kev ntxiv rau kev ua kom lub cev muaj zog ntxiv, Dorrestein hais tias, yuav yog ntxiv cov ntsiab lus siab ntawm ions xws li sodium los yog poov tshuaj thiab tsis tshua muaj siab ntawm cov hlau txaus siab. “Nws dhau los ua kev sim sib tw. Nws yuav qhia rau koj paub, OK, cov molecule no nyob rau hauv cov xwm txheej no muaj ntau lub tswv yim los khi sodium thiab potassium los yog ib qho tshwj xeeb hlau uas koj tau ntxiv, "Dorrestein hais. "Peb tuaj yeem ua rau ntau yam hlau sib txawv ib txhij, thiab peb tuaj yeem nkag siab qhov kev nyiam thiab kev xaiv hauv cov ntsiab lus ntawd."

Hauv kab lis kev cai rho tawm los ntawm Escherichia coli, cov kws tshawb fawb tau txheeb xyuas cov khoom sib txuas ntawm cov hlau xws li yersiniabactin thiab aerobactin. Nyob rau hauv rooj plaub ntawm yersiniabactin, lawv pom tias nws tuaj yeem khi zinc.

Cov kws tshawb fawb tau txheeb xyuas cov hlau-binding compounds hauv cov qauv ua complex li yaj cov organic teeb meem los ntawm dej hiav txwv. Dorrestein hais tias "Qhov ntawd yog ib qho ntawm cov qauv uas nyuaj tshaj plaws uas kuv tau pom dua," "Nws yog tej zaum nyuaj li, yog tias tsis complex dua, roj av." Txoj kev tau txheeb xyuas domoic acid raws li tooj liab-binding molecule thiab qhia tias nws khi Cu2 + raws li ib tug dimer.

"Ib txoj hauv kev omics los txheeb xyuas tag nrho cov hlau-binding metabolites hauv ib qho qauv yog qhov tseem ceeb heev vim tias qhov tseem ceeb ntawm kev lom neeg hlau chelation," Oliver Baars, uas kawm txog hlau-binding metabolites tsim los ntawm cov nroj tsuag thiab microbes ntawm North Carolina State University, sau nyob rau hauv ib tug email.

"Dorrestein thiab cov neeg ua haujlwm ua haujlwm muab qhov zoo nkauj, xav tau ntau, tshuaj xyuas kom zoo dua qhov kev ua haujlwm ntawm lub cev ntawm cov hlau ions hauv lub xovtooj tuaj yeem yog," Albert JR Heck, tus thawj coj hauv zej zog kev tshuaj ntsuam xyuas ntawm Utrecht University, sau hauv email. "Ib kauj ruam tom ntej yuav yog kom rho tawm cov metabolites nyob rau hauv cov xwm txheej ib txwm muaj los ntawm lub xovtooj ntawm tes thiab faib cov no hauv cov xwm txheej ib txwm muaj, kom pom cov metabolites uas nqa cov cellular hlau ions endogenous."

Post lub sij hawm: Dec-23-2021